Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / In Vitro Assessment of Cardiac Function Using…
JoVE Journal
Medicine

Ce contenu est en libre accès.

JoVE Journal Medicine
In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes
 

In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

Article DOI: 10.3791/60427-v • 08:19 min • June 22nd, 2020
June 22nd, 2020 •
*1, *1, 1, 1, 1, 2, 1
1Unidade de Investigação Cardiovascular, Departamento de Cirurgia e Fisiologia, Faculdade de Medicina, Universidade do Porto, 2Department of Physiology, Kilimanjaro Christian Medical University College
* These authors contributed equally

Chapitres

résumé
Automatic Translation

This protocol aims to describe step-by-step the technique of extraction and assessment of cardiac function using skinned cardiomyocytes. This methodology allows measurement and acutemodulation of myofilament function using small frozen biopsies that can be collected from different cardiac locations, from mice to men.

Tags

In Vitro Assessment Cardiac Function Skinned Cardiomyocytes Pathophysiology Correlation In Vivo Parameters Animal Models Humans Advantage Deep Myofilament Function Small Biopsies Frozen Samples Therapeutic Interventions Experiment Cardiomyocyte Extraction Select Glue Activate Size Striation Shape Temperature Testing Apparatus Force Transducer Motor Myocardial Sample Petri Dish Relax ISO Solution Scalpel Tissue Pieces Cell Damage Cut Pipette Tip Solution And Tissue Fragments Potter-Elvehjem Glass Grinder Mechanically Disrupt Tissue
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter