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In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes
Chapters
- 00:04Introduction
- 01:00Skinned Cardiomyocyte Extraction and Permeabilization
- 02:46Skinned Cardiomyocyte Selection and Gluing
- 04:03Active, Passive and Calcium (Ca2+) Sensitivity Force Measurements
- 05:58Results: Representative Skinned Cardiomyocyte Assessment
- 07:36Conclusion
This protocol aims to describe step-by-step the technique of extraction and assessment of cardiac function using skinned cardiomyocytes. This methodology allows measurement and acutemodulation of myofilament function using small frozen biopsies that can be collected from different cardiac locations, from mice to men.
