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在人类多能干细胞中使用CRISPR-CAS9生成定义的基因组修饰

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遗传学

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JoVE 杂志遗传学

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

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在人类多能干细胞中使用CRISPR-CAS9生成定义的基因组修饰

8,014 Views

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09:04 min

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September 25, 2019

DOI:

10.3791/60085-v

DOI:

10.3791/60085-v

•

09:04 min

September 25, 2019

•

3 Views

Fabian L. Cardenas-Diaz*¹, Jean Ann Maguire*¹, Paul Gadue^1,2,3, Deborah L. French^1,2,3

¹Center for Cellular and Molecular Therapeutics,The Children’s Hospital of Philadelphia, ²Department of Pathology and Laboratory Medicine,The Children’s Hospital of Philadelphia, ³Department of Pathology and Laboratory Medicine,University of Pennsylvania

Summary

该协议提供了一种方法，促进在人类多能干细胞中使用CRISPR-CAS9生成定义的异质核苷酸或同源核苷酸变化。

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