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Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin

Mechanistic Insight into the Development of TNBS-Mediated Intestinal Fibrosis and Evaluating the Inhibitory Effects of Rapamycin

DOI:
10.3791/60067-v

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10:21 min

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September 12, 2019

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, , , ,
1Department of Molecular and Cellular Physiology,Albany Medical College, 2The IBD Center, Division of Gastroenterology, Department of Medicine,Albany Medical College, 3Department of Geriatrics, School of Medicine and Health Science,University of North Dakota, 4Department of Neuroscience and Experimental Therapeutics,Albany Medical College

Chapters

  • 00:04Title
  • 00:52Induction of TNBS Fibrosis and Rapamycin Treatment in Mice
  • 01:58Isolation of Intestinal Lamina Propria and Purification of Cx3Cr1+ Mononuclear Phagocytes
  • 04:01Purification of Mononuclear Phagocytes from the Collected Cells Purification: Magnetic Purification
  • 04:51Purification of Mononuclear Phagocytes from the Collected Cells Purification: FACS Sorting
  • 05:39Flow Cytometry Analysis
  • 07:39Results: Analysis of TNBS-Mediated Intestinal Fibrosis
  • 09:27Conclusion

Summary

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In this study, we describe a detailed procedure of TNBS-mediated intestinal fibrosis, which exhibits comparable pathophysiology to Crohn's fibrosis. We also discuss this approach in light of rapamycin facilitated inhibitory effects on intestinal fibrosis.

Tags

TNBSIntestinal FibrosisCrohn’s DiseaseRapamycinExtracellular MatrixColitisLamina PropriaCollagenaseDNASE1

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