Journal
/
发育生物学
/
Performing Human Skeletal Muscle Xenografts in Immunodeficient Mice
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Performing Human Skeletal Muscle Xenografts in Immunodeficient Mice

Performing Human Skeletal Muscle Xenografts in Immunodeficient Mice

DOI:
10.3791/59966-v

•

07:48 min

•

September 16, 2019

•

, , ,
1Graduate Program in Cellular and Molecular Medicine,Johns Hopkins University School of Medicine, 2Department of Neurology,Johns Hopkins University School of Medicine, 3Kennedy Krieger Institute, 4Department of Neuroscience,Johns Hopkins University School of Medicine

Chapters

  • 00:04Title
  • 00:35Surgical Preparation
  • 01:48Xenograft Surgery
  • 03:52Xenograft Harvest
  • 05:43Results: Representative Human Skeletal Muscle Xenograft Analyses
  • 07:12Conclusion

Summary

自动翻译

自动翻译

Complex human diseases can be challenging to model in traditional laboratory model systems. Here, we describe a surgical approach to model human muscle disease through the transplantation of human skeletal muscle biopsies into immunodeficient mice.

Tags

Keywords: Human Skeletal Muscle XenograftsImmunodeficient MiceMuscle Disease ModelingPreclinical Therapeutic TestingHuman Muscle Cell BiologyRare Or Acquired Muscle DiseasesMuscle BiopsyNOD-Rag-gamma MouseTibialis AnteriorExtensor Digitorum LongusPeroneus LongusSurgical Procedure

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/59966?article_type=v

Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
check_url/cn/59966?article_type=v

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
check_url/cn/59966?article_type=v

Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
check_url/cn/59966?article_type=v

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection
check_url/cn/59966?article_type=v

Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle
check_url/cn/59966?article_type=v

Application of Chronic Stimulation to Study Contractile Activity-induced Rat Skeletal Muscle Phenotypic Adaptations
check_url/cn/59966?article_type=v

Identification of Skeletal Muscle Satellite Cells by Immunofluorescence with Pax7 and Laminin Antibodies
check_url/cn/59966?article_type=v

In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae
check_url/cn/59966?article_type=v

Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure
check_url/cn/59966?article_type=v

Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants

Read Article