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淀粉样蛋白纤维蛋白的相互作用和脑线粒体的膜渗透
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生物化学
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Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils
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淀粉样蛋白纤维蛋白的相互作用和脑线粒体的膜渗透

5,786 Views

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00:15 min

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September 28, 2019

DOI:

10.3791/59883-v

DOI:
10.3791/59883-v

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00:15 min
September 28, 2019

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4 Views
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1Department of Biological Sciences,Institute for Advanced Studies in Basic Sciences (IASBS), Zanjan, 45137-66731, Iran

Summary

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这里提供了一个协议，用于研究从不同组织和大脑不同区域分离的来自不同组织和不同区域的线粒体的不同肽和蛋白质的原生形态、前纤维和成熟的淀粉样纤维之间的相互作用。

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MitochondriaAmyloid FibrilsAlpha-synucleinMembrane PermeabilizationNeurodegenerative DiseasesBrainIsolationHomogenizationCentrifugationDensity GradientMyelinMitochondrial Fraction

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Cite this Article

Zadali, R., Ghareghozloo, E. R., Ramezani, M., Hassani, V., Rafiei, Y., Chiyaneh, S. M., Meratan, A. A. Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils. J. Vis. Exp. (151), e59883, doi:10.3791/59883 (2019).

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