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从富足人类记忆B细胞群中选择和恢复具有所需特异性的抗体的单细胞筛选方法
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免疫与感染
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JoVE 杂志 免疫与感染
Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
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从富足人类记忆B细胞群中选择和恢复具有所需特异性的抗体的单细胞筛选方法

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00:09 min

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August 22, 2019

DOI:

10.3791/59809-v

DOI:
10.3791/59809-v

•

00:09 min
August 22, 2019

•

16 Views
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1World Without Disease Accelerator,Janssen Pharmaceutical Companies of Johnson and Johnson, 2Janssen Prevention Center,Janssen Pharmaceutical Companies of Johnson and Johnson

Summary

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BSelex 方法用于识别和从人类外周血单核细胞中识别和回收单个抗原特异性抗体，将流式细胞学与单细胞 PCR 和克隆相结合。

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Perry, S. T., Keogh, E., Morton, M., Koudstaal, W., Pascual, G. Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations. J. Vis. Exp. (150), e59809, doi:10.3791/59809 (2019).

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