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Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection
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Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection

Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection

9,181 Views

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05:31 min

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September 14, 2019

DOI:

10.3791/59610-v

DOI:
10.3791/59610-v

•

05:31 min
September 14, 2019

•

28 Views
, ,
1Hubrecht Institute, Oncode Institute, Royal Netherlands Academy of Arts and Sciences (KNAW),UMC Utrecht, 2Veterinary Microbiology and Pathology, College of Veterinary Medicine,Washington State University, 3Janssen Pharmaceutica

Summary

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We describe protocols to prepare oocysts and purify sporozoites for studying infection of human intestinal and airway organoids by Cryptosporidium parvum. We demonstrate the procedures for microinjection of parasites into the intestinal organoid lumen and immunostaining of organoids. Finally, we describe the isolation of generated oocysts from the organoids.

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Dutta, D., Heo, I., O'Connor, R. Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection. J. Vis. Exp. (151), e59610, doi:10.3791/59610 (2019).

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