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通过微注射研究3D组织衍生的人体器官培养系统中的隐孢子虫感染

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JoVE 杂志免疫与感染

Studying Cryptosporidium Infection in 3D Tissue-derived Human Organoid Culture Systems by Microinjection

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通过微注射研究3D组织衍生的人体器官培养系统中的隐孢子虫感染

9,167 Views

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05:31 min

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September 14, 2019

DOI:

10.3791/59610-v

DOI:

10.3791/59610-v

•

05:31 min

September 14, 2019

•

28 Views

Devanjali Dutta¹, Inha Heo^1,3, Roberta O’Connor²

¹Hubrecht Institute, Oncode Institute, Royal Netherlands Academy of Arts and Sciences (KNAW),UMC Utrecht, ²Veterinary Microbiology and Pathology, College of Veterinary Medicine,Washington State University, ³Janssen Pharmaceutica

Summary

我们描述了用于制备卵母细胞和纯化孢子菌的方案，用于研究隐孢子虫的人类肠道和气道器官的感染。我们演示了将寄生虫微注射到肠道器官流明和有机体免疫染色的程序。最后，我们描述了从器官中分离生成的卵母细胞。

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