Journal
/
发育生物学
/
X-ray Diffraction of Intact Murine Skeletal Muscle as a Tool for Studying the Structural Basis of Muscle Disease
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
X-ray Diffraction of Intact Murine Skeletal Muscle as a Tool for Studying the Structural Basis of Muscle Disease

X-ray Diffraction of Intact Murine Skeletal Muscle as a Tool for Studying the Structural Basis of Muscle Disease

6,805 Views

•

08:26 min

•

July 18, 2019

DOI:

10.3791/59559-v

DOI:
10.3791/59559-v

•

08:26 min
July 18, 2019

•

4 Views
,
1BioCAT, Dept. of Biological Sciences,Illinois Institute of Technology

Summary

Automatically generated

We present detailed protocols for performing small-angle X-ray diffraction experiments using intact mouse skeletal muscles. With the wide availability of transgenic mouse models for human diseases, this experimental platform can form a useful test bed for elucidating the structural basis of genetic muscle diseases

Explore More Videos

X-ray DiffractionMurine Skeletal MuscleMuscle DiseaseStructural BasisPhysiological ConditionsGenetically Modified ModelsMyopathiesExtensor Digitorum LongusSoleus MuscleDissectionRinger’s SolutionGastrocnemius MuscleTibialis Anterior Muscle

Read Article

Cite this Article

Ma, W., Irving, T. C. X-ray Diffraction of Intact Murine Skeletal Muscle as a Tool for Studying the Structural Basis of Muscle Disease. J. Vis. Exp. (149), e59559, doi:10.3791/59559 (2019).

Download .ris file

Copy

Share Video

.

Copy