Journal
/
免疫与感染
/
Isolação e avaliação quantitativa de pilhas da escova dos Tracheas do rato
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas

Isolação e avaliação quantitativa de pilhas da escova dos Tracheas do rato

6,992 Views

•

10:25 min

•

June 12, 2019

DOI:

10.3791/59496-v

DOI:
10.3791/59496-v

•

10:25 min
June 12, 2019

•

3 Views
, , ,
1Division of Rheumatology, Allergy and Immunology, Brigham and Women’s Hospital,Harvard Medical School

Tags

Tracheal Brush CellsEpithelial IsolationPapain DigestionCholine AcetyltransferaseFluorescent Reporter MiceTranscriptional AnalysisRNA SequencingUpper Airway EpitheliumDispaseDNase ITyrode’s BufferFACS BufferEuthanasia

Read Article

Cite this Article

Ualiyeva, S., Yoshimoto, E., Barrett, N. A., Bankova, L. G. Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas. J. Vis. Exp. (148), e59496, doi:10.3791/59496 (2019).

Download .ris file

Copy

Share Video

.

Copy