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마우스 기관에서 브러시 셀의 격리 및 정량 평가
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JoVE 杂志 免疫与感染
Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas
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마우스 기관에서 브러시 셀의 격리 및 정량 평가

6,992 Views

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10:25 min

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June 12, 2019

DOI:

10.3791/59496-v

DOI:
10.3791/59496-v

•

10:25 min
June 12, 2019

•

3 Views
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1Division of Rheumatology, Allergy and Immunology, Brigham and Women’s Hospital,Harvard Medical School

Tags

Tracheal Brush CellsEpithelial IsolationPapain DigestionCholine AcetyltransferaseFluorescent Reporter MiceTranscriptional AnalysisRNA SequencingUpper Airway EpitheliumDispaseDNase ITyrode’s BufferFACS BufferEuthanasia

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Ualiyeva, S., Yoshimoto, E., Barrett, N. A., Bankova, L. G. Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas. J. Vis. Exp. (148), e59496, doi:10.3791/59496 (2019).

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