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Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas
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Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas

Isolation and Quantitative Evaluation of Brush Cells from Mouse Tracheas

DOI:
10.3791/59496-v

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10:25 min

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June 12, 2019

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, , ,
1Division of Rheumatology, Allergy and Immunology, Brigham and Women’s Hospital,Harvard Medical School

Chapters

  • 00:04Title
  • 01:24Preparation of Reagents
  • 02:34Dissection of Mouse Trachea
  • 04:47Tracheal Epithelial Digestion
  • 08:31Results: Analysis of the Isolated Brush Cells from Mouse Tracheas
  • 09:57Conclusion

Summary

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自动翻译

Brush cells are rare cholinergic chemosensory epithelial cells found in the naïve mouse trachea. Due to their limited numbers, ex vivo evaluation of their functional role in airway immunity and remodeling is challenging. We describe a method for isolation of tracheal brush cells by flow cytometry.

Tags

Tracheal Brush CellsEpithelial IsolationPapain DigestionCholine AcetyltransferaseFluorescent Reporter MiceTranscriptional AnalysisRNA SequencingUpper Airway EpitheliumDispaseDNase ITyrode’s BufferFACS BufferEuthanasia

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