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一种通用嵌套逆转录酶链反应检测裂解病毒的评价
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Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
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一种通用嵌套逆转录酶链反应检测裂解病毒的评价

8,246 Views

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08:10 min

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May 02, 2019

DOI:

10.3791/59428-v

DOI:
10.3791/59428-v

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08:10 min
May 02, 2019

•

4 Views
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1Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine,Academy of Military Medical Sciences, 2Jilin Agricultural University, 3Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

Summary

Automatically generated

一种泛裂解病毒嵌套逆转录聚合酶链反应已开发出来, 专门检测所有已知的溶酶病毒。利用不同动物物种的狂犬病脑样本进行验证, 表明该方法具有与金标准荧光抗体检测相当的敏感性和特异性, 可用于常规狂犬病诊断。

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Wang, Y., Xu, W., Guo, H., Gong, W., He, B., Tu, Z., Tu, C., Feng, Y. Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses. J. Vis. Exp. (147), e59428, doi:10.3791/59428 (2019).

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