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병원 균, 공생, 그리고 호스트 창 자 상피 사이 상호 작용을 공부 하는 인간 Colonoid Monolayers
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免疫与感染
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JoVE 杂志 免疫与感染
Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
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병원 균, 공생, 그리고 호스트 창 자 상피 사이 상호 작용을 공부 하는 인간 Colonoid Monolayers

8,520 Views

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07:20 min

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April 09, 2019

DOI:

10.3791/59357-v

DOI:
10.3791/59357-v

•

07:20 min
April 09, 2019

•

2 Views
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1Department of Internal Medicine,University of New Mexico Health Science Center, 2Department of Medicine, Division of Gastroenterology & Hepatology,Johns Hopkins University School of Medicine

Tags

Colonoid MonolayersIntestinal EpitheliumHost-pathogen InteractionMucus BiologyMicrobiomeInflammatory Bowel Disease3D Organoid CultureEx Vivo StudiesHarvesting SolutionCell SuspensionDMEM/F12 MediumCell PlatingExpansion Medium

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Cite this Article

In, J. G., Foulke-Abel, J., Clarke, E., Kovbasnjuk, O. Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium. J. Vis. Exp. (146), e59357, doi:10.3791/59357 (2019).

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