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Monocouches de Colonoid humain pour étudier les Interactions entre les pathogènes, germes commensaux et hôte épithélium Intestinal
JoVE 杂志
免疫与感染
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JoVE 杂志 免疫与感染
Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
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Monocouches de Colonoid humain pour étudier les Interactions entre les pathogènes, germes commensaux et hôte épithélium Intestinal

8,520 Views

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07:20 min

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April 09, 2019

DOI:

10.3791/59357-v

DOI:
10.3791/59357-v

•

07:20 min
April 09, 2019

•

2 Views
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1Department of Internal Medicine,University of New Mexico Health Science Center, 2Department of Medicine, Division of Gastroenterology & Hepatology,Johns Hopkins University School of Medicine

Tags

Colonoid MonolayersIntestinal EpitheliumHost-pathogen InteractionMucus BiologyMicrobiomeInflammatory Bowel Disease3D Organoid CultureEx Vivo StudiesHarvesting SolutionCell SuspensionDMEM/F12 MediumCell PlatingExpansion Medium

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In, J. G., Foulke-Abel, J., Clarke, E., Kovbasnjuk, O. Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium. J. Vis. Exp. (146), e59357, doi:10.3791/59357 (2019).

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