Study on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea (<em>Camellia Sinensis </em>L.) Leaves

Weiting Jiao; Guoqin Ge; Rimao Hua; Jun Sun; Yeyun Li; Ruyan Hou

doi:10.3791/59312

/

Study on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea (Camellia Sinensis L.) Leaves

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生物化学
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JoVE 杂志生物化学

Study on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea (Camellia Sinensis L.) Leaves

Study on the Metabolism of Six Systemic Insecticides in a Newly Established Cell Suspension Culture Derived from Tea (Camellia Sinensis L.) Leaves

DOI:

10.3791/59312-v

•

10:35 min

•

June 15, 2019

•

Weiting Jiao*^1,2, Guoqin Ge*¹, Rimao Hua, Jun Sun, Yeyun Li, Ruyan Hou

¹State Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology,Anhui Province Key Lab of Analysis and Detection for Food Safety, ²School of Resource & Environment, Anhui Agricultural University,Key Laboratory of Agri-food Safety of Anhui Province

Chapters

00:00Title
01:46Tea Callus Culture
02:46Tea Cell Suspension Culture
03:43TTC Assay of Cell Viability
04:25Treatment and Sampling of Tea Cell Suspension Cultures with Insecticides
06:24Sample Preparation of Insecticides in Intact Tea Plant
06:56Instrument Analysis
07:54Results
10:02Conclusion

Summary

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This work presents a protocol for establishing a cell suspension culture derived from tea (Camellia sinensis L.) leaves that can be used to study the metabolism of external compounds that can be taken up by the whole plant, such as insecticides.

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