需要订阅 JoVE 才能查看此. 登录或开始免费试用。
1Department of Biological Sciences,University of Alberta, 2Women & Children’s Health Research Institute,University of Alberta, 3Division of Anatomy, Department of Surgery,University of Alberta, 4Department of Cell Biology,University of Alberta, 5Department of Medical Genetics,University of Alberta, 6Neuroscience and Mental Health Research Institute,University of Alberta
Chapters
- 00:04Title
- 00:28Superior Ocular Sulcus (SOS) Visualization
- 03:15Whole-Mount Immunofluorescent Laminin Staining
- 06:04Results: Representative Zebrafish SOS Imaging
- 07:31Conclusion
Aquí, presentamos una serie estandarizada de protocolos para observar el surco ocular superior, una estructura recientemente identificadas, conservadas evolutivamente en el ojo vertebrado. Con larvas de pez cebra, demostramos técnicas necesarias para identificar los factores que contribuyen a la formación y cierre del surco ocular superior.
