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多夏巴氏杆菌自主表达采用除抑制技术

JoVE 杂志
生物工程

This content is Free Access.

JoVE 杂志生物工程

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

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多夏巴氏杆菌自主表达采用除抑制技术

13,047 Views

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05:30 min

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January 23, 2019

DOI:

10.3791/58589-v

DOI:

10.3791/58589-v

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05:30 min

January 23, 2019

•

9 Views

Jasmin Elgin Fischer¹, Anna-Maria Hatzl², Astrid Weninger², Christian Schmid², Anton Glieder^1,2

¹bisy e.U., ²Institute of Molecular Biotechnology,Technical University of Graz

Summary

该方法首次介绍了在毕赤酵母 (komagataella phaffii)高效诱导无甲醇重组蛋白生产的可靠实验程序, 并采用了碳源调节启动子 p _dc和 p_df 在小规模试验中, 在可在线监测培养参数的同时, 可采用恒定的甘油饲料。

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