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凝胶神经元全细胞斑块夹的急性脊髓切片的制备

JoVE 杂志
神经科学

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JoVE 杂志神经科学

Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons

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凝胶神经元全细胞斑块夹的急性脊髓切片的制备

DOI:

10.3791/58479-v

•

08:30 min

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January 18, 2019

•

Mengye Zhu, Daying Zhang, Sicong Peng, Nana Liu, Jing Wu, Haixia Kuang, Tao Liu³

¹Department of Pain,First Affiliated Hospital of Nanchang University, ²Department of Pediatrics,First Affiliated Hospital of Nanchang University, ³Center for Laboratory Medicine,First Affiliated Hospital of Nanchang University

Chapters

00:04Title
00:39Preparation of Micropipettes and Agar Block
01:30Spinal Cord Dissection and Slicing
03:46Whole-cell Patch-clamp Recordings
06:57Results: Voltage-clamp Recordings in SG Neurons
08:03Conclusion

Summary

自动翻译

在这里, 我们描述了由体外脊髓切片中的明胶 (sg) 神经元制成的全细胞膜片夹记录的基本步骤。该方法可对 sg 神经元的固有膜特性、突触传递和形态表征进行研究。

Tags

Acute Spinal Cord Slices Whole-cell Patch-clamp Recording Substantia Gelatinosa Neurons Sensory Transmission Nociceptive Regulation Chronic Pain Agar Block Sucrose-based ACSF Transcardial Perfusion Spinal Cord Extraction Lumbosacral Enlargement Meninges Removal Pia Arachnoid Membrane Ventral/dorsal Roots Transverse Slices Parasagittal Slices Vibratome

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