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Vorbereitung der akuten Rückenmark Scheiben auf Whole-Cell Patch-Clamp-Aufnahme in den Neuronen der Substantia Gelatinosa
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神经科学
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JoVE 杂志 神经科学
Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons
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Vorbereitung der akuten Rückenmark Scheiben auf Whole-Cell Patch-Clamp-Aufnahme in den Neuronen der Substantia Gelatinosa

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08:30 min

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January 18, 2019

DOI:

10.3791/58479-v

DOI:
10.3791/58479-v

•

08:30 min
January 18, 2019

•

8 Views
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1Department of Pain,First Affiliated Hospital of Nanchang University, 2Department of Pediatrics,First Affiliated Hospital of Nanchang University, 3Center for Laboratory Medicine,First Affiliated Hospital of Nanchang University

Summary

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Hier beschreiben wir die wesentlichen Schritte für die ganze Zelle Patch-Clamp-Aufnahmen, die von Neuronen der Substantia Gelatinosa (SG) in der in-vitro- Rückenmark-Slice. Diese Methode ermöglicht die innere Membran Eigenschaften, synaptische Übertragung und morphologische Charakterisierung der SG Neuronen untersucht werden.

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Zhu, M., Zhang, D., Peng, S., Liu, N., Wu, J., Kuang, H., Liu, T. Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons. J. Vis. Exp. (143), e58479, doi:10.3791/58479 (2019).

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