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生物化学
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Studying Protein Import into Chloroplasts Using Protoplasts
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生物化学
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JoVE 杂志 生物化学
Studying Protein Import into Chloroplasts Using Protoplasts

Studying Protein Import into Chloroplasts Using Protoplasts

DOI:
10.3791/58441-v

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06:29 min

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December 10, 2018

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, ,
1Division of Integrative Biosciences and Biotechnology,Pohang University of Science and Technology, 2Department of Life Sciences,Pohang University of Science and Technology

Chapters

  • 00:04Title
  • 00:33Protoplast Isolation
  • 02:19Protoplast Transformation using Polyethylene Glycol
  • 03:53Analysis of the Protein Import into Chloroplasts
  • 05:10Results: Fluorescence Microscopy and Immunoblotting to Analyze the Import of Proteins into Chloroplasts
  • 06:07Conclusion

Summary

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Here we describe a protocol to express proteins into protoplasts by using PEG-mediated transformation method. The method provides easy expression of proteins of interest, and efficient investigation of protein localization and the import process for various experimental conditions in vivo.

Tags

Protein ImportChloroplastsProtoplastsCell BiologyProtein InputIn Vivo ConditionsEnzyme SolutionSucrose SolutionW5 SolutionMANG SolutionPEG SolutionCentrifugationIncubationPipetteDNA Transformation

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