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Studying Protein Import into Chloroplasts Using Protoplasts
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Studying Protein Import into Chloroplasts Using Protoplasts

Studying Protein Import into Chloroplasts Using Protoplasts

9,377 Views

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06:29 min

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December 10, 2018

DOI:

10.3791/58441-v

DOI:
10.3791/58441-v

•

06:29 min
December 10, 2018

•

13 Views
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1Division of Integrative Biosciences and Biotechnology,Pohang University of Science and Technology, 2Department of Life Sciences,Pohang University of Science and Technology

Summary

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Here we describe a protocol to express proteins into protoplasts by using PEG-mediated transformation method. The method provides easy expression of proteins of interest, and efficient investigation of protein localization and the import process for various experimental conditions in vivo.

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Cite this Article

Lee, J., Kang, H., Hwang, I. Studying Protein Import into Chloroplasts Using Protoplasts. J. Vis. Exp. (142), e58441, doi:10.3791/58441 (2018).

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