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Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding
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Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

7,712 Views

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09:12 min

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November 14, 2018

DOI:

10.3791/58341-v

DOI:
10.3791/58341-v

•

09:12 min
November 14, 2018

•

2 Views
, , , , , ,
1Department of Psychiatry,Columbia University, 2Department of Pathology & Cell Biology,Columbia University Medical Center, 3Department of Psychiatry, Division of Molecular Imaging and Neuropathology,New York State Psychiatric Institute, 4EB Sciences

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Here, we present a protocol to isolate brain nuclei in the neonatal rat brain in conjunction with first colostrum feeding. This technique allows the study of nutrient insufficiency stress in the brain as modulated by enterocyte signaling.

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Klein, B. Y., Tamir, H., Anwar, M., Ludwig, R. J., Kaidbey, J. H., Glickstein, S. B., Welch, M. G. Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding. J. Vis. Exp. (141), e58341, doi:10.3791/58341 (2018).

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