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Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding
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Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

DOI:
10.3791/58341-v

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09:12 min

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November 14, 2018

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, , , , , ,
1Department of Psychiatry,Columbia University, 2Department of Pathology & Cell Biology,Columbia University Medical Center, 3Department of Psychiatry, Division of Molecular Imaging and Neuropathology,New York State Psychiatric Institute, 4EB Sciences

Chapters

  • 00:04Title
  • 00:48Tissue Preparation and Protein Extraction
  • 02:46In-capillary Protein Measurement Sample Preparation and Signal Protein Analysis
  • 06:32Results: Representative Signaling Protein Marker Expression in Primed and Unprimed Neonatal Rat Brain Tissue
  • 08:26Conclusion

Summary

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Here, we present a protocol to isolate brain nuclei in the neonatal rat brain in conjunction with first colostrum feeding. This technique allows the study of nutrient insufficiency stress in the brain as modulated by enterocyte signaling.

Tags

OxytocinBrain NucleiNeonatal RatColostrum FeedingCellular StressInflammationPost-natal DevelopmentMilk SucklingBrain PunchesProtein ExtractionProtein Measurement

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