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Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding
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JoVE Journal 免疫学と感染
Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

Assessing Cellular Stress and Inflammation in Discrete Oxytocin-secreting Brain Nuclei in the Neonatal Rat Before and After First Colostrum Feeding

DOI:
10.3791/58341-v

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09:12 min

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November 14, 2018

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, , , , , ,
1精神医学科,Columbia University, 2Department of Pathology & Cell Biology,Columbia University Medical Center, 3Department of Psychiatry, Division of Molecular Imaging and Neuropathology,New York State Psychiatric Institute, 4EB Sciences

章

  • 00:04標題
  • 00:48Tissue Preparation and Protein Extraction
  • 02:46In-capillary Protein Measurement Sample Preparation and Signal Protein Analysis
  • 06:32Results: Representative Signaling Protein Marker Expression in Primed and Unprimed Neonatal Rat Brain Tissue
  • 08:26Conclusion

概要

自動翻訳

自動翻訳

Here, we present a protocol to isolate brain nuclei in the neonatal rat brain in conjunction with first colostrum feeding. This technique allows the study of nutrient insufficiency stress in the brain as modulated by enterocyte signaling.

タグ

OxytocinBrain NucleiNeonatal RatColostrum FeedingCellular StressInflammationPost-natal DevelopmentMilk SucklingBrain PunchesProtein ExtractionProtein Measurement

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