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改进杆状病毒表达载体生产昆虫细胞分泌植物蛋白
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生物化学
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Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
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改进杆状病毒表达载体生产昆虫细胞分泌植物蛋白

11,129 Views

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09:14 min

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August 20, 2018

DOI:

10.3791/58283-v

DOI:
10.3791/58283-v

•

09:14 min
August 20, 2018

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6 Views
, ,
1Department of Molecular and Structural Biochemistry, College of Agriculture and Life Sciences,North Carolina State University

Summary

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在这里, 我们提出了利用昆虫细胞和杆状病毒蛋白表达系统来产生大量植物分泌蛋白的蛋白质结晶的协议。用 GP67 或昆虫 hemolin 信号肽对昆虫细胞中植物蛋白分泌表达进行了改进, 并对杆状病毒表达载体进行了修正。

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Cite this Article

Chakraborty, S., Trihemasava, K., Xu, G. Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells. J. Vis. Exp. (138), e58283, doi:10.3791/58283 (2018).

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