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改进杆状病毒表达载体生产昆虫细胞分泌植物蛋白
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Modifying Baculovirus Expression Vectors to Produce Secreted Plant Proteins in Insect Cells
DOI:

09:14 min

August 20, 2018

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Chapters

  • 00:04Title
  • 00:32Modification of a Baculovirus Expression Vector with either the GP67 Signal Peptideor the Insect Hemolin Signal Peptidefor Plant Protein Secretion Expression
  • 02:24Production and Amplification of Baculovirus Constructs Harboring the Recombinant Protein Expression Cassette
  • 07:02Results: Analysis of Plant Proteins Secreted by Insect Cells
  • 07:57Conclusion

Summary

自动翻译

在这里, 我们提出了利用昆虫细胞和杆状病毒蛋白表达系统来产生大量植物分泌蛋白的蛋白质结晶的协议。用 GP67 或昆虫 hemolin 信号肽对昆虫细胞中植物蛋白分泌表达进行了改进, 并对杆状病毒表达载体进行了修正。

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