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1Center for Applied Tissue Engineering and Regenerative Medicine,Munich University of Applied Sciences, 2FG Protein Biochemistry & Cellular Microbiology,Munich University of Applied Sciences, 3Center for Nano Science,Ludwig-Maximilians-Universität München, 4Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie,University Medical Center Göttingen
Chapters
- 00:04Title
- 00:39Immobilization of Proteins via Functional Silane Coupling Agents
- 04:42Atomic Force Microscopy Based Single Molecule Force Spectroscopy
- 08:38Results: Single Force Spectroscopy
- 10:07Conclusion
Det här protokollet beskriver kovalent immobilisering av proteiner med heterobifunctional silan kontaktmedel kiseloxid ytor avsedda för den atomic force microscopy baserat enda molekyl kraft spektroskopin som exemplifieras av den växelverkan av RrgA (pilus-1 tip adhesin av S. pneumoniae) med Fibronektin.
