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单分子力谱中蛋白质共价固定化的研究

JoVE 杂志
生物化学

This content is Free Access.

JoVE 杂志生物化学

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

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单分子力谱中蛋白质共价固定化的研究

10,606 Views

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11:13 min

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August 20, 2018

DOI:

10.3791/58167-v

DOI:

10.3791/58167-v

•

11:13 min

August 20, 2018

•

2 Views

Tanja D. Becke^1,2,3, Stefan Ness², Stefanie Sudhop^1,3, Hermann E. Gaub³, Markus Hilleringmann², Arndt F. Schilling*⁴, Hauke Clausen-Schaumann*^1,3

¹Center for Applied Tissue Engineering and Regenerative Medicine,Munich University of Applied Sciences, ²FG Protein Biochemistry & Cellular Microbiology,Munich University of Applied Sciences, ³Center for Nano Science,Ludwig-Maximilians-Universität München, ⁴Klinik für Unfallchirurgie, Orthopädie und Plastische Chirurgie,University Medical Center Göttingen

Summary

本协议描述了 heterobifunctional 硅烷偶联剂在硅氧化物表面上的共价固定化, 其作用是以原子力显微镜为基础的单分子力光谱学为例。RrgA (pilus-1 尖黏附)与纤连蛋白的相互作用。

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