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Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn (Zea mays) As an Exemplar
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环境科学
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JoVE 杂志 环境科学
Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn (Zea mays) As an Exemplar

Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn (Zea mays) As an Exemplar

DOI:
10.3791/58164-v

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07:19 min

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August 06, 2018

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, , , , ,
1Department of Entomology,Texas A&M University, 2Department of Entomology,University of Minnesota, 3Department of Entomology,University of Kentucky

Chapters

  • 00:04Title
  • 00:52Soluble Protein Assay
  • 03:58Digestible Carbohydrate Assay
  • 05:52Results: Differences in Soluble Protein Content Observed Between Geographic Regions and Tissue Types
  • 06:42Conclusion

Summary

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自动翻译

The protocols described herein provide a clear and approachable methodology for measuring soluble protein and digestible (non-structural) carbohydrate content in plant tissues. The ability to quantify these two plant macronutrients has significant implications for advancing the fields of plant physiology, nutritional ecology, plant-herbivore interactions and food-web ecology.

Tags

Keywords: Plant Soluble ProteinDigestible CarbohydrateZea MaysMacronutrientsFood WebsSodium HydroxideCentrifugationTrichloroacetic AcidAcetoneProtein Quantification96-well Plate

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