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CRISPR Guide RNA kloning for pattedyr systemer
1Wyss Institute for Biologically Inspired Engineering,Harvard University, 2Department of Genetics,Harvard Medical School, 3Department of Pathology,Massachusetts General Hospital, 4Institute for Medical Engineering & Science,Massachusetts Institute of Technology, 5Synthetic Biology Center,Massachusetts Institute of Technology, 6Department of Biological Engineering,Massachusetts Institute of Technology, 7Broad Institute
Chapters
- 00:04Title
- 00:25Preparation of the Primers and Expression Vectors
- 02:05Ligating the Annealed gRNA Oligonucleotides
- 02:59Transformation of E. coli
- 04:03PCR Analysis
- 05:35Results: CRISPR Guided RNA Cloning
- 06:23Conclusion
