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Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
JoVE 杂志
化学
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JoVE 杂志 化学
Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry

DOI:
10.3791/57966-v

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07:33 min

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October 15, 2018

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, , ,
1Department of Chemistry,King’s College London

Chapters

  • 00:04Title
  • 00:45Native MS Acquisition and Analysis for Investigating Protein Complexes and Protein-Ligand Complexes
  • 03:51Acquiring and Analyzing IM-MS
  • 04:34In-Solution Disruption of Protein Complexes for Native MS and IM-MS Led Structure Determination and Investigating Protein Complex Stability using Collision Induced Unfolding
  • 05:45Results: Integrative Structural Mass Spectrometry of Protein Architectures and Protein-Ligand Complexes
  • 07:05Conclusion

Summary

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Mass spectrometry (MS) has emerged as an important tool for the investigation of structure and dynamics of macromolecular assemblies. Here, we integrate MS-based approaches to interrogate protein complex formation and ligand binding.

Tags

Keywords: Protein ArchitectureProtein-ligand ComplexStructural Mass SpectrometryNative Mass SpectrometryBiomolecular AssembliesHeterogeneous Protein SamplesOligomeric StateNanoelectrosprayAmmonium AcetateIon Mobility SeparationCapillary HolderNano-flow Gas PressureCapillary VoltageSampling ConeSource OffsetSource TemperatureCone Gas FlowTrap Collision EnergyTrap Voltage

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