00:36Plant Excavation, Soil Removal, and Bulk Soil Collection
01:46Root and Rhizosphere Collection
02:44Root Surface Sterilization
03:54Processing Rhizosphere Samples
05:02DNA Extraction and Soil Analysis Processing
06:33DNA Extraction in 96-well Format
07:45Rhizosphere Sample Loading
08:47Results: Visualization and Statistical Methods Highlight Differences in Microbial Community Composition and Diversity Between Sample Types and Grass Species
Udgravning af planternes rødder fra feltet samt behandling af prøver i endosphere, rhizosfære og jord er beskrevet i detaljer, herunder DNA-ekstraktion og data analysemetoder. Dette papir er beregnet til at aktivere andre laboratorier til at anvende disse metoder til undersøgelse af jordbundens, endosphere og rhizosfære microbiomes.