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Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo
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发育生物学
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JoVE 杂志 发育生物学
Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo

Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo

DOI:
10.3791/57900-v

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08:23 min

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November 02, 2018

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, , ,
1CNRS, Institut de Biologie du Développement de Marseille,Aix-Marseille Université

Chapters

  • 00:04Title
  • 00:49Preparing the Optical Tweezers
  • 02:01Interfacing the Instrument
  • 02:52Calibration of the Optical Trap
  • 03:45Position Interpolation with Image Analysis
  • 04:30Mounting Drosophila Embryos
  • 05:12Trapping Experiment In Vivo
  • 06:06Mechanical Measurements
  • 06:44Results: Interface Deflection Imposed by Sinusoidal Movement
  • 07:50Conclusion

Summary

自动翻译

自动翻译

We present a setup of optical tweezers coupled to a light sheet microscope, and its implementation to probe cell mechanics without beads in the Drosophila embryo.

Tags

Optical TweezersDrosophila EmbryoCell-cell ContactsLive ImagingLight Sheet MicroscopyFluorescent BeadsLaserQT CreatorMicroRheoData Acquisition BoardOptical Shutter Interface

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