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Where You Cut Matters: A Dissection and Analysis Guide for the Spatial Orientation of the Mouse Retina from Ocular Landmarks
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Where You Cut Matters: A Dissection and Analysis Guide for the Spatial Orientation of the Mouse Retina from Ocular Landmarks

Where You Cut Matters: A Dissection and Analysis Guide for the Spatial Orientation of the Mouse Retina from Ocular Landmarks

13,430 Views

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08:42 min

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August 04, 2018

DOI:

10.3791/57861-v

DOI:
10.3791/57861-v

•

08:42 min
August 04, 2018

•

17 Views
, , , ,
1Department of Biology,The University of Akron, 2Department of Cell and Developmental Biology,University of Colorado Denver

Summary

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This protocol provides a comprehensive dissection and analysis guide for the use of deep ocular landmarks, s-opsin immunohistochemistry, Retistruct, and custom code to accurately and reliably orient the isolated mouse retina in anatomical space.

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Mouse RetinaOcular LandmarksDissection ProtocolSpatial OrientationRetinal TopographyDorsalVentralNasalTemporalCorneal BurnRectus MusclesChoroid FissureS-opsin GradientEnucleationRetinal IsolationSclera RemovalIrisLensVitreousRight EyeLeft Eye

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Sondereker, K. B., Stabio, M. E., Jamil, J. R., Tarchick, M. J., Renna, J. M. Where You Cut Matters: A Dissection and Analysis Guide for the Spatial Orientation of the Mouse Retina from Ocular Landmarks. J. Vis. Exp. (138), e57861, doi:10.3791/57861 (2018).

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