Journal
/
工程学
/
Méthode fondée sur un embout de la Pipette pour semis de cellules aux plateformes microfluidiques Droplet
JoVE 杂志
工程学
This content is Free Access.
JoVE 杂志 工程学
A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms
Please note that all translations are automatically generated. Click here for the English version.

Méthode fondée sur un embout de la Pipette pour semis de cellules aux plateformes microfluidiques Droplet

10,690 Views

•

06:50 min

•

February 11, 2019

DOI:

10.3791/57848-v

DOI:
10.3791/57848-v

•

06:50 min
February 11, 2019

•

7 Views
, , , ,
1Department of Biomedical Engineering and Institute for Complex Molecular Systems, Laboratory of Immunoengineering,Eindhoven University of Technology, 2Institute for Immunity, Transplantation, and Infection, Beckman Center,Stanford University

Summary

Automatically generated

Cet article présente un protocole pour l’amorçage rare population de cellules à l’aide de pointes de pipette à dispositifs microfluidiques gouttelette afin d’offrir une plus grande efficacité d’encapsulation des cellules en gouttelettes.

Explore More Videos

Pipette-tipCell SeedingDroplet MicrofluidicsSystems ImmunologyCellular HeterogeneityCommunicationRare CellsEncapsulationAqueous Droplet GenerationSurfactantFluorinated OilMineral OilPDMSSyringe PumpSample SolutionOil PhaseRPMI Medium

Read Article

Cite this Article

Sinha, N., Subedi, N., Wimmers, F., Soennichsen, M., Tel, J. A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms. J. Vis. Exp. (144), e57848, doi:10.3791/57848 (2019).

Download .ris file

Copy

Share Video

.

Copy