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婆罗洲 "爆炸蚂蚁" (蚁科昆虫) 对下颌腺储层含量的分离--gc-ms 和 MetaboliteDetector Volatilome 分析
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August 26, 2018
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1Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln),University of Natural Resources and Life Sciences Vienna (BOKU), Austria, 2Institute of Chemical, Environmental and Biological Engineering,TU Vienna, Austria, 3Department of Computational Biology of Infection Research,Helmholtz Centre for Infection Research, Germany, 4Institute for Biochemistry, Biotechnology and Bioinformatics,University of Technology (TU) Braunschweig, Germany, 52nd Zoological Department,Natural History Museum Vienna, Austria, 6Chemical Sciences,Universiti Brunei Darussalam, Brunei, 7Environmental and Life Sciences,Universiti Brunei Darussalam, Brunei
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对蚂蚁Colobopsis explodens的小工人进行了解剖, 以分离出其肥厚下颌腺储层中储存的蜡样含量, 用于后续溶剂萃取和气相色谱-质谱分析。本文还介绍了利用开源软件 MetaboliteDetector 对挥发性成分进行标注和识别的方法。
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Hoenigsberger, M., Kopchinskiy, A. G., Parich, A., Hiller, K., Laciny, A., Zettel, H., Lim, L. B., Salim, K. A., Druzhinina, I. S., Schuhmacher, R. Isolation of Mandibular Gland Reservoir Contents from Bornean 'Exploding Ants' (Formicidae) for Volatilome Analysis by GC-MS and MetaboliteDetector. J. Vis. Exp. (138), e57652, doi:10.3791/57652 (2018).
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