Journal
/
发育生物学
/
Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries

Whole Mount Immunofluorescence and Follicle Quantification of Cultured Mouse Ovaries

DOI:
10.3791/57593-v

•

08:15 min

•

May 02, 2018

•

, ,
1Department of Biomedical Sciences,Cornell University, 2Department of Molecular Biology and Genetics,Cornell University

Chapters

  • 00:04Title
  • 00:50Preparation for Ovary Dissection and Culture
  • 02:13Ovary Dissection and Organ Culture
  • 03:34Tissue Fixation
  • 04:12Whole Mount Immunofluorescence
  • 05:42Ovary Clearing for Imaging
  • 06:37Results: Immunofluorescence Imaging of Cleared Ovaries
  • 07:23Conclusion

Summary

自动翻译

自动翻译

Here, we present a protocol to quantify follicles in cultured ovaries of young mice without serial sectioning. Using whole organ immunofluorescence and tissue clearing, physical sectioning is replaced with optical sectioning. This method of sample preparation and visualization maintains organ integrity and facilitates automated quantification of specific cells.

Tags

Keywords: Whole Mount ImmunofluorescenceFollicle QuantificationCultured Mouse OvariesReproductive BiologyThree-dimensional ArchitectureDissectionIncubatorOvary Culture MediaImagingAutomated Analysis

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/57593?article_type=v

Culture and Co-Culture of Mouse Ovaries and Ovarian Follicles
check_url/cn/57593?article_type=v

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
check_url/cn/57593?article_type=v

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program
check_url/cn/57593?article_type=v

Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts
check_url/cn/57593?article_type=v

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
check_url/cn/57593?article_type=v

Whole-mount Clearing and Staining of Arabidopsis Flower Organs and Siliques
check_url/cn/57593?article_type=v

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
check_url/cn/57593?article_type=v

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence
check_url/cn/57593?article_type=v

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence
check_url/cn/57593?article_type=v

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Read Article