Waiting
登录处理中...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Identifying Transcription Factor Olig2 Genomic…
JoVE Journal
Neuroscience

需要订阅 JoVE 才能查看此 内容. 登录或开始免费试用。

JoVE Journal Neuroscience
Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis
 

Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis

Article DOI: 10.3791/57547-v • 12:29 min • April 16th, 2018
April 16th, 2018 •
1, 1, 1, 1
1The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center

章节

总结概括
Automatic Translation

Here we present a protocol which is designed to analyze the genome-wide binding of the oligodendrocyte transcription factor 2 (Olig2) in acutely purified brain oligodendrocyte precursor cells (OPCs) by performing low-cell chromatin immunoprecipitation (ChIP), library preparation, high-throughput sequencing and bioinformatic data analysis.

Tags

Keywords: Transcription Factor Olig2 PDGFR-α+ Cells ChIP-seq Low-cell ChIP-seq Oligodendrocyte Precursor Cells Immunopanning Genome-wide Transcriptional Regulation Epigenetic Mechanisms Cell Differentiation Cell Development
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter