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Um isoinokosterone baseado no Receptor Singular Gene interruptor para deliberada expressão do Transgene com robustez, reversibilidade e insignificante Leakiness
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遗传学
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An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

Um isoinokosterone baseado no Receptor Singular Gene interruptor para deliberada expressão do Transgene com robustez, reversibilidade e insignificante Leakiness

6,353 Views

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06:21 min

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May 07, 2018

DOI:

10.3791/57494-v

DOI:
10.3791/57494-v

•

06:21 min
May 07, 2018

•

4 Views
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1Department of Biological Sciences, College of Bioscience and Biotechnology,Chungnam National University, 2Department of Pharmacology, College of Medicine,Chungnam National University, 3Department of Nursing,Gwangju Women’s University

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Aqui, apresentamos um protocolo para a modulação da expressão do transgene usando pEUI(+) singular gene interruptor por tebufenozide tratamento.

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Ecdysone ReceptorGene SwitchTransgene ExpressionTebufenozideHEK 293 CellsEGFPANKRD13ATransfectionImmunoblotting

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Lee, S., Won, M., Hwang, R. H., Hur, G. M., Ro, H. An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness. J. Vis. Exp. (135), e57494, doi:10.3791/57494 (2018).

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