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Imágenes de células vivas de la segregación de cromosomas durante la Mitosis
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发育生物学
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Live Cell Imaging of Chromosome Segregation During Mitosis
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Imágenes de células vivas de la segregación de cromosomas durante la Mitosis

9,070 Views

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06:39 min

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March 14, 2018

DOI:

10.3791/57389-v

DOI:
10.3791/57389-v

•

06:39 min
March 14, 2018

•

7 Views
, ,
1Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research,US Food and Drug Administration, 2Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research,US Food and Drug Administration

Summary

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Este protocolo describe un método sencillo y conveniente para marcar y visualizar energizados cromosomas en las células mitotic usando la etiqueta Histone2B-GFP BacMam 2.0 y un sistema de microscopia confocal spinning disc.

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Cite this Article

Varadkar, P., Takeda, K., McCright, B. Live Cell Imaging of Chromosome Segregation During Mitosis. J. Vis. Exp. (133), e57389, doi:10.3791/57389 (2018).

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