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CRISPR 介导的小脑颗粒细胞在宫内电穿孔基因转移中的功能分析损失
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09:39 min
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June 09, 2018
DOI:
1Division of Molecular Neurogenetics,German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, 2Division of Molecular Genetics,German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 3Hopp-Children’s Cancer Center at the NCT Heidelberg (KiTZ), 4Division of Pediatric Neurooncology,German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 5Department of Pediatric Hematology and Oncology,Heidelberg University Hospital
Summary
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传统的功能丧失基因的研究使用挖空动物往往是昂贵和耗时。基于电穿孔的 CRISPR 介导体细胞诱变是了解体内基因功能的有力工具。在这里, 我们报告了一个方法来分析的淘汰赛表型的小脑增殖细胞。
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Cite this Article
Feng, W., Herbst, L., Lichter, P., Pfister, S. M., Liu, H., Kawauchi, D. CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer. J. Vis. Exp. (136), e57311, doi:10.3791/57311 (2018).
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