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96井格式人诱导多潜能干细胞源神经元高速拉伸损伤的方法
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format
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96井格式人诱导多潜能干细胞源神经元高速拉伸损伤的方法

6,642 Views

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08:35 min

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April 20, 2018

DOI:

10.3791/57305-v

DOI:
10.3791/57305-v

•

08:35 min
April 20, 2018

•

2 Views
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1Department of Neurosurgery,NorthShore University HealthSystem, 2Midwestern University/Chicago College of Osteopathic Medicine, 3Independent Contractor

Tags

Human Induced Pluripotent Stem Cell-derived NeuronsHigh-speed Stretch Injury96-well FormatNeurotrauma ModelPhenotypic Drug DiscoveryAPTES CoatingSilicone MembranePlate Fabrication ClampStretching DeviceMotion ControlIn Vitro Neurotrauma

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Phillips, J. K., Sherman, S. A., Oungoulian, S. R., Finan, J. D. Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format. J. Vis. Exp. (134), e57305, doi:10.3791/57305 (2018).

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