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Membran proteinlerinin Hofstede deterjan duyarlı tespiti
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Detection of Detergent-sensitive Interactions Between Membrane Proteins
DOI:

10:09 min

March 07, 2018

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Chapters

  • 00:04Title
  • 01:05Handling of the Peptide and Cells
  • 03:02Binding of His-tagged Proteins to the HisPur Cobalt Beads
  • 04:39Binding of the Peptide to the His-tagged Protein Immobilized on the Beads
  • 05:59Elution from Cobalt Beads
  • 07:07Electrophoresis, Western Blotting, and Analysis of Results
  • 07:58Results: Use of Immunoblot Assay to Study the Interaction between Sortilin Immobilized on Cobalt Beads and myc-tagged Peptide
  • 09:18Conclusion

Summary

自动翻译

Biz algılama deterjan-duyarlı sıralama reseptör, sortilin, GLUT4, glukoz taşıyıcı protein ilk luminal döngü için bağlama örnek olarak kullanarak zar proteinleri arasında bir köprü işlevi için bir iletişim kuralı tanımlamak.

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