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Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity
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JoVE 杂志 神经科学
Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity

Neuron-Macrophage Co-cultures to Activate Macrophages Secreting Molecular Factors with Neurite Outgrowth Activity

DOI:
10.3791/56920-v

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08:52 min

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March 30, 2018

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, ,
1Department of Brain Science,Ajou University School of Medicine, 2Neuroscience Graduate Program, Department of Biomedical Sciences,Ajou University School of Medicine, 3Department of Neurology,Ajou University School of Medicine

Chapters

  • 00:04Title
  • 01:06Culture Preparation of Dissociated Adult DRG Neuron
  • 03:41Co-culture of Primary Peritoneal Macrophages on A Cell Culture Insert
  • 05:47Neurite Outgrowth Assay with Collected Conditioned Medium
  • 07:05Results: Representative Results of Neurite Outgrowth Assay Using Conditioned Medium Obtained from Various Conditions
  • 07:55Conclusion

Summary

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The current protocol presents experimental procedures to stimulate cultured macrophages to be endowed with capacity to release molecular factors that promote neurite outgrowth. Treatment of cAMP to the neuron-macrophage co-cultures induces the macrophages to produce conditioned medium that possesses strong neurite outgrowth activity.

Tags

Neuron-macrophage Co-cultureMacrophage ActivationNeurite OutgrowthRegenerationDRGCollagenaseCell CultureDMEM

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