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Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis
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发育生物学
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JoVE 杂志 发育生物学
Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis

Zika Virus Infection of Cultured Human Fetal Brain Neural Stem Cells for Immunocytochemical Analysis

DOI:
10.3791/56917-v

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10:27 min

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February 05, 2018

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, ,
1Department of Neuroscience and Cell Biology,University of Texas Medical Branch, 2Department of Neurology,Johns Hopkins University, 3Beijing Institute for Brain Disorders,Capital Medical University

Chapters

  • 00:04Title
  • 00:57hNSC Passage
  • 04:50Plating Adherent hNSC for Validation
  • 06:02Zika Virus Infection
  • 07:37Results: Human Fetal Brain Neural Stem Cell Infection with Zika Virus
  • 09:03Conclusion

Summary

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This article details the methods that are used to expand human fetal brain neural stem cells in culture, as well as how to differentiate them into various neuronal subtypes and astrocytes, with an emphasis on the use of neural stem cells to study Zika virus infection.

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Zika VirusHuman Fetal Brain Neural Stem CellsImmunocytochemical AnalysisIn VitroNeural Stem Cell DifferentiationInnate Immune ResponseTranslational ApplicationNeurological DeficitsCell CultureCell PassageTrypsinDNaseCentrifugation

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