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Metabole Labeling en profilering van overdracht RNAs met behulp van Macroarrays
JoVE 杂志
遗传学
This content is Free Access.
JoVE 杂志 遗传学
Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays
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Metabole Labeling en profilering van overdracht RNAs met behulp van Macroarrays

5,531 Views

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10:56 min

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January 16, 2018

DOI:

10.3791/56898-v

DOI:
10.3791/56898-v

•

10:56 min
January 16, 2018

•

1 Views
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1Laboratory of tRNA Biology, Department of Biology,College of Charleston, 2Department of Biochemistry and Molecular Biology,MUSC, 3Department of Biology,College of Charleston

Summary

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We beschrijven een originele transfer-RNA analyse platform genaamd SPOt (gestroomlijnde Platform voor de waarneming van tRNA). Ter plaatse gelijktijdig maatregelen cellulaire niveaus van alle tRNAs in biologische monsters, in slechts drie stappen en in minder dan 24 uur.

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Cite this Article

Emetu, S., Troiano, M., Goldmintz, J., Tomberlin, J., Grelet, S., Howe, P. H., Korey, C., Geslain, R. Metabolic Labeling and Profiling of Transfer RNAs Using Macroarrays. J. Vis. Exp. (131), e56898, doi:10.3791/56898 (2018).

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