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Retrograd Neuroanatomical sporing af Phrenic motoriske neuroner i mus
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JoVE 杂志 神经科学
Retrograde Neuroanatomical Tracing of Phrenic Motor Neurons in Mice

Retrograd Neuroanatomical sporing af Phrenic motoriske neuroner i mus

DOI:
10.3791/56758-v

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09:05 min

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February 22, 2018

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, , , ,
1URVI, NARILIS,Université de Namur, 2URPhyM, NARILIS,Université de Namur

Chapters

  • 00:05Title
  • 01:23Preparations
  • 02:22Intrapleural Injections Using Transdiaphragmatic Approach
  • 04:48Intrapleural Injection using Transthoracic Approach and Post-operative Care
  • 06:38Results: Labeling Neurons with CTB after a C4 Spinal Contusion
  • 08:01Conclusion

Summary

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自动翻译

Her, beskriver vi en protokol til at identificere phrenic motoriske neuroner i mus efter intrapleural levering af fluorophore konjugeret kolera toksin subunit beta. To teknikker er sammenlignet for at injicere den pleural hulrum: transdiaphragmatic versus transthoracic tilgange.

Tags

Keywords: Retrograde Neuroanatomical TracingPhrenic Motor NeuronsMiceIntrapleural DeliveryFluorophore-conjugated Cholera Toxin Subunit BetaTransdiaphragmatic ApproachTransthoracic ApproachNeuromuscular JunctionsPleural SpaceSterile SurgeryAnesthesiaSkin PreparationCTB SolutionMicrosyringeLaparotomy

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