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发育生物学
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Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis
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发育生物学
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JoVE 杂志 发育生物学
Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis

Methods for Analyzing the Impacts of Natural Uranium on In Vitro Osteoclastogenesis

DOI:
10.3791/56499-v

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09:51 min

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January 30, 2018

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, , , , ,
1UMR E-4320 TIRO-MATOs CEA/DRF/BIAM,Université Nice Sophia-Antipolis, 2UMR 7272 Institut de Chimie de Nice CNRS,Université Nice Sophia-Antipolis, 3CEA, Direction de la Recherche Fondamentale (DRF),Biosciences and Biotechnologies Institute (BIAM)

Chapters

  • 00:05Title
  • 00:38RAW 264.7 Differentiation in the Presence of Uranyl Ions [U(VI)]
  • 03:14Osteoclast Size and Morphology Analyses
  • 05:27Osteoclast Function in the Presence of U(VI)
  • 08:30Results: Representative Effects of U(VI) on Osteoclast Size and Function
  • 09:34Conclusion

Summary

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Uranium is known to affect bone metabolism. Here, we present a protocol aimed at investigating the effect of natural uranium exposure on the viability, the differentiation, and the function of osteoclasts, the cells in charge of bone resorption.

Tags

OsteoclastogenesisUranium ExposureRAW 264.7 CellsCell CultureCell Seeding DensityUranium Salt SolutionRANKLOsteoclast DifferentiationOsteoclast FunctionEnvironmental ToxicityBone PhysiologyBone Metabolism

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