Waiting
登录处理中...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / High-resolution Confocal Imaging of the…
JoVE Journal
Neuroscience

这篇 内容 是开放权限的.

JoVE Journal Neuroscience
High-resolution Confocal Imaging of the Blood-brain Barrier
 

High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis

Article DOI: 10.3791/56407-v • 10:30 min • November 16th, 2017
November 16th, 2017 •
1, 1
1Roche Pharma Research and Early Development (pRED), Neuroimmunology, Roche Innovation Center Basel

章节

总结概括
Automatic Translation

Here we present a microscopy-based protocol for high-resolution imaging and a three-dimensional reconstruction of the mouse neurovascular unit and blood-brain barrier using brain free-floating sections. This method allows for the visualization, analysis, and quantification of intracellular organelles at the BBB.

Tags

High-resolution Confocal Imaging Blood-brain Barrier Microscopy-based Protocol 3-dimensional Reconstruction Immunofluorescence Staining Subcellular Organelles Neurovascular Unit Transcytosis Laser Scanning Confocal Microscopy Image Analysis Endothelial Cells
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter