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Live Imaging efterfulgt af enkelt celle inddeling hen til dataskærm cellebiologi og afstamning Progression af flere neurale populationer
1Biochemistry and Molecular Biology Department, Faculty of Veterinary medicine,Complutense University, 2University Institute for Neurochemistry Research (IUIN), 3Instituto de Investigación Sanitaria del Hospital Clínico San Carlos (IdISSC), 4Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg/Munich, Germany Physiological Genomics, Biomedical Center,Ludwig-Maximilians University Munich, 5Toxicology and Pharmacology Department, Faculty of Veterinary medicine,Complutense University, 6Brain Institute,Federal University of Rio Grande do Norte, 7Department of Biosystems Science and Engineering,Eidgenössische Technische Hochschule (ETH) Zurich
Chapters
- 00:05Title
- 01:14Live Imaging by Time-lapse Video-microscopy
- 04:02Post-imaging Immunocytochemistry, Data Collection, and Processing
- 06:38Single-cell Tracking
- 08:56Results: Example of aNSCs Isolated from the SEZ and Analyzed by Live Imaging and Single Cell Tracking
- 10:17Conclusion
Et robust protokol til at overvåge neurale populationer af time-lapse video-mikroskopi efterfulgt af software-baserede efterbehandling er beskrevet. Denne metode repræsenterer et kraftfuldt værktøj til at identificere biologiske hændelser i en valgte befolkning under live imaging eksperimenter.
